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The N-terminus of the RNA polymerase from infectious pancreatic necrosis virus is the determinant of genome attachment.

Abstract:

The RNA-dependent RNA polymerase VP1 of infectious pancreatic necrosis virus (IPNV) is a single polypeptide responsible for both viral RNA transcription and genome replication. Sequence analysis identifies IPNV VP1 as having an unusual active site topology. We have purified, crystallized and solved the structure of IPNV VP1 to 2.3 Å resolution in its apo form and at 2.2 Å resolution bound to the catalytically-activating metal magnesium. We find that recombinantly-expressed VP1 is highly activ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.ppat.1002085

Authors


Graham, SC More by this author
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Institution:
University of Oxford
More by this author
Institution:
University of Oxford
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
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Academy of Finland More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PLoS pathogens Journal website
Volume:
7
Issue:
6
Pages:
e1002085
Publication date:
2011-06-05
DOI:
EISSN:
1553-7374
ISSN:
1553-7366
URN:
uuid:db35b697-ed34-4dad-9eae-ff24194eff08
Source identifiers:
163991
Local pid:
pubs:163991

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