Observation of fast release of NO from ferrous d₁ haem allows formulation of a unified reaction mechanism for cytochrome cd₁ nitrite reductases.

Journal article

Cytochrome cd1 nitrite reductase is a haem-containing enzyme responsible for the reduction of nitrite into NO, a key step in the anaerobic respiratory process of denitrification. The active site of cytochrome cd1 contains the unique d1 haem cofactor, from which NO must be released. In general, reduced haems bind NO tightly relative to oxidized haems. In the present paper, we present experimental evidence that the reduced d1 haem of cytochrome cd1 from Paracoccus pantotrophus releases NO rapid...

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Authors: Rinaldo, S; Sam, K; Castiglione, N; Stelitano, V; Arcovito, A

• Publication status: Published
• Journal: Biochemical journal
• Volume: 435
• Issue: 1
• Pages: 217-225
• Publication date: 2011-04-01
• DOI: 10.1042/bj20101615
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Paracoccus pantotrophus; Models, Molecular; Nitrite Reductases; Kinetics; Biocatalysis; Myoglobin; Oxidation-Reduction; Photolysis; Heme; Denitrification; Cytochromes; Bacterial Proteins; Nitric Oxide; Apoproteins