- Abstract:
-
Using 5-fluoroorotic acid (5-FOA) as a positive selection system we isolated mutants of Mucor circinelloides altered in the pyrimidine biosynthetic pathway. These mutants were found to be deficient either in orotidine-5'-monophosphate decarboxylase (OMPdecase), or in orotate phosphoribosyltransferase (OPRTase) activity. Complementation tests among mutants lacking OPRTase activity classified them into three groups, thus suggesting the possibility of interallelic complementation. To investigate...
Expand abstract - Publisher copy:
- 10.1007/s004380050893
-
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- Journal:
- Molecular and general genetics : MGG
- Volume:
- 260
- Issue:
- 2-3
- Pages:
- 251-260
- Publication date:
- 1998-11-05
- DOI:
- EISSN:
-
1432-1874
- ISSN:
-
0026-8925
- URN:
-
uuid:da724bf1-b234-4885-9899-4a4a57f23fe8
- Source identifiers:
-
354448
- Local pid:
- pubs:354448
- Language:
- English
- Keywords:
- Copyright date:
- 1998
Journal article
Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides.
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