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Fluorescence in situ hybridisation of multiple probes on a single microscope slide.

Abstract:

We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a...

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Publication status:
Published

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Publisher copy:
10.1093/nar/22.18.3689

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Ishikawa-Brush, Y More by this author
Southern, EM More by this author
Journal:
Nucleic acids research
Volume:
22
Issue:
18
Pages:
3689-3692
Publication date:
1994-09-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:da51797d-65c3-40e0-b171-f35d20a97585
Source identifiers:
35750
Local pid:
pubs:35750

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