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Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families

Abstract:

Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial libraries. We developed a highly sensitive and adaptable donor/acceptor pair that can be used to investigate the substrate specificity of cysteine proteases, serine proteases and metalloproteinases....

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/srep43135

Authors


Kasperkiewicz, P More by this author
Rutkowska-Wlodarczyk, I More by this author
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Polish National Science Centre More from this funder
Iuventus Plus More from this funder
Foundation for Polish Science More from this funder
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Publisher:
Nature Publishing Group Publisher's website
Journal:
Scientific Reports Journal website
Volume:
7
Pages:
43135
Publication date:
2017-02-05
Acceptance date:
2017-01-19
DOI:
ISSN:
2045-2322
Pubs id:
pubs:724037
URN:
uri:d9c60119-8c17-4f9d-bbbf-a3b3b25a031f
UUID:
uuid:d9c60119-8c17-4f9d-bbbf-a3b3b25a031f
Local pid:
pubs:724037

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