Journal article
Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families
- Abstract:
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Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial libraries. We developed a highly sensitive and adaptable donor/acceptor pair that can be used to investigate the substrate specificity of cysteine proteases, serine proteases and metalloproteinases....
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
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(pdf, 1.5MB)
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- Publisher copy:
- 10.1038/srep43135
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Authors
Funding
Polish National Science Centre
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Iuventus Plus
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Foundation for Polish Science
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Bibliographic Details
- Publisher:
- Nature Publishing Group Publisher's website
- Journal:
- Scientific Reports Journal website
- Volume:
- 7
- Pages:
- 43135
- Publication date:
- 2017-02-05
- Acceptance date:
- 2017-01-19
- DOI:
- ISSN:
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2045-2322
- Pubs id:
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pubs:724037
- URN:
-
uri:d9c60119-8c17-4f9d-bbbf-a3b3b25a031f
- UUID:
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uuid:d9c60119-8c17-4f9d-bbbf-a3b3b25a031f
- Local pid:
- pubs:724037
Terms of use
- Copyright holder:
- Itoh et al
- Copyright date:
- 2017
- Notes:
- © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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