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Characterization of the single-stranded DNA binding protein pV(VGJΦ) of VGJΦ phage from Vibrio cholerae.

Abstract:

pV(VGJΦ), a single-stranded DNA binding protein of the vibriophage VGJΦ was subject to biochemical analysis. Here, we show that this protein has a general affinity for single-stranded DNA (ssDNA) as documented by Electrophoretic Mobility Shift Assay (EMSA). The apparent molecular weight of the monomer is about 12.7kDa as measured by HPLC-SEC. Moreover, isoelectrofocusing showed an isoelectric point for pV(VGJΦ) of 6.82 pH units. Size exclusion chromatography in 150mM NaCl, 50mM sodium phospha...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Condensed Matter Physics
Role:
Author
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Journal:
Biochimica et biophysica acta
Volume:
1814
Issue:
9
Pages:
1107-1112
Publication date:
2011-09-05
DOI:
EISSN:
1878-1454
ISSN:
0006-3002
URN:
uuid:d8b4f158-41cd-4836-afaf-f71d577dbc8a
Source identifiers:
156193
Local pid:
pubs:156193

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