Characterization of the single-stranded DNA binding protein pV(VGJΦ) of VGJΦ phage from Vibrio cholerae.

Journal article

pV(VGJΦ), a single-stranded DNA binding protein of the vibriophage VGJΦ was subject to biochemical analysis. Here, we show that this protein has a general affinity for single-stranded DNA (ssDNA) as documented by Electrophoretic Mobility Shift Assay (EMSA). The apparent molecular weight of the monomer is about 12.7kDa as measured by HPLC-SEC. Moreover, isoelectrofocusing showed an isoelectric point for pV(VGJΦ) of 6.82 pH units. Size exclusion chromatography in 150mM NaCl, 50mM sodium phospha...

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Authors: Falero, A; Caballero, A; Trigueros, S; Pérez, C; Campos, J

• Publication status: Published
• Journal: Biochimica et biophysica acta
• Volume: 1814
• Issue: 9
• Pages: 1107-1112
• Publication date: 2011-09-01
• DOI: 10.1016/j.bbapap.2011.04.013
• EISSN: 1878-1454
• ISSN: 0006-3002
• Language: English
• Keywords: Molecular Weight; Isoelectric Point; Vibrio cholerae; Viral Proteins; Protein Multimerization; DNA-Binding Proteins; Computational Biology; Bacteriophages