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Defining the limits of single-molecule FRET resolution in TIRF microscopy.

Abstract:

Single-molecule FRET (smFRET) has long been used as a molecular ruler for the study of biology on the nanoscale (∼2-10 nm); smFRET in total-internal reflection fluorescence (TIRF) Förster resonance energy transfer (TIRF-FRET) microscopy allows multiple biomolecules to be simultaneously studied with high temporal and spatial resolution. To operate at the limits of resolution of the technique, it is essential to investigate and rigorously quantify the major sources of noise and error; we used t...

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Publication status:
Published

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Publisher copy:
10.1016/j.bpj.2010.09.005

Authors


Holden, SJ More by this author
Hohlbein, J More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
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Journal:
Biophysical journal
Volume:
99
Issue:
9
Pages:
3102-3111
Publication date:
2010-11-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:d89dceda-6a3b-4371-83d0-d2e4771d071d
Source identifiers:
93185
Local pid:
pubs:93185

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