nNOS gene transfer to RVLM improves baroreflex function in rats with chronic heart failure.

Journal article

We hypothesized that gene transfer of neuronal nitric oxide synthase (nNOS) into the rostral ventrolateral medulla (RVLM) improves baroreflex function in rats with chronic heart failure (CHF). Six to eight weeks after coronary artery ligation, rats showed hemodynamic signs of CHF. A recombinant adenovirus, either Ad.nNOS or Ad.beta-Gal, was transfected into the RVLM. nNOS expression in the RVLM was confirmed by Western blot analysis, NADPH-diaphorase, and immunohistochemical staining. We stud...

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Authors: Wang, Y; Patel, K; Cornish, K; Channon, K; Zucker, I

• Publication status: Published
• Journal: American journal of physiology. Heart and circulatory physiology
• Volume: 285
• Issue: 4
• Pages: H1660-H1667
• Publication date: 2003-10-01
• DOI: 10.1152/ajpheart.00239.2003
• EISSN: 1522-1539
• ISSN: 0363-6135
• Language: English
• Keywords: Histocytochemistry; Arteries; Medulla Oblongata; Sympathetic Nervous System; Chronic Disease; Nitric Oxide Synthase; Immunohistochemistry; Rats, Sprague-Dawley; Kidney; Blotting, Western; Male; Heart Rate; Nitric Oxide Synthase Type I; beta-Galactosidase; Baroreflex; NADPH Dehydrogenase; Animals; Hemodynamics; Gene Transfer Techniques; Cardiac Output, Low; Rats