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Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley.

Abstract:

Barley powdery mildew, Erysiphe graminis f.sp. hordei, is an obligate biotrophic pathogen and as such cannot complete its life cycle without a living host. The inability to transform this fungus and manipulate its genome has constrained research towards understanding its life cycle and pathogenicity. Here we describe an in planta transformation system based on delivery of DNA using a gold-particle gun and selection using benomyl or bialaphos. Using this method, we consistently obtained stable...

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Publication status:
Published

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Publisher copy:
10.1038/72666

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
Journal:
Nature biotechnology
Volume:
18
Issue:
2
Pages:
205-207
Publication date:
2000-02-05
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
URN:
uuid:d7a0deaf-de2e-4b18-8ca7-97cd1613eb21
Source identifiers:
31614
Local pid:
pubs:31614

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