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Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements.

Abstract:

Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to exposure with NO, the active site Fe(II) is octahedrally coordinated by a water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the side-chains of an aspartyl and two histidinyl residues. NO binds to th...

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Publication status:
Published

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Authors


McKinnon, CH More by this author
Clifton, IJ More by this author
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Journal:
FEBS letters
Volume:
517
Issue:
1-3
Pages:
7-12
Publication date:
2002-04-05
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
URN:
uuid:d79cc411-57f6-4552-aa06-9bd60bfdeace
Source identifiers:
32126
Local pid:
pubs:32126

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