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Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above

Abstract:

The instability of DNA triplexes particularly at neutral pH and above severely limits their applications. Here, we demonstrate that the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs) resolves this problem. The stabilising effects are additive; multiple TO units produce nanomolar duplex binding and triplex stability can surpass that of the underlying duplex. In one example, a TFO containing three TO units increased the t...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1039/c8sc02418a

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Subgroup:
Organic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Subgroup:
Organic Chemistry
Role:
Author
ORCID:
0000-0002-6538-3036
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Grant:
Marie Sklodowska Curie agreement 642023 (H2020-MSCA-ITN-2014- 642023)
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
9
Issue:
39
Pages:
Article: 7681
Publication date:
2018-08-01
Acceptance date:
2018-07-30
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pubs id:
pubs:935218
URN:
uri:d79cbd11-6811-4778-9e7c-ff2743d78d39
UUID:
uuid:d79cbd11-6811-4778-9e7c-ff2743d78d39
Local pid:
pubs:935218

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