- Abstract:
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The instability of DNA triplexes particularly at neutral pH and above severely limits their applications. Here, we demonstrate that the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs) resolves this problem. The stabilising effects are additive; multiple TO units produce nanomolar duplex binding and triplex stability can surpass that of the underlying duplex. In one example, a TFO containing three TO units increased the t...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's Version
- Files:
-
-
(pdf, 828.2KB)
-
- Publisher copy:
- 10.1039/c8sc02418a
-
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- Grant:
- Marie Sklodowska Curie agreement 642023 (H2020-MSCA-ITN-2014- 642023)
- Publisher:
- Royal Society of Chemistry Publisher's website
- Journal:
- Chemical Science Journal website
- Volume:
- 9
- Issue:
- 39
- Pages:
- Article: 7681
- Publication date:
- 2018-08-01
- Acceptance date:
- 2018-07-30
- DOI:
- EISSN:
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2041-6539
- ISSN:
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2041-6520
- Pubs id:
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pubs:935218
- URN:
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uri:d79cbd11-6811-4778-9e7c-ff2743d78d39
- UUID:
-
uuid:d79cbd11-6811-4778-9e7c-ff2743d78d39
- Local pid:
- pubs:935218
- Copyright holder:
- Royal Society of Chemistry
- Copyright date:
- 2018
- Notes:
- © The Royal Society of Chemistry 2018. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Journal article
Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above
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+ European Union Horizon 2020 research and innovation programme
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