Journal article
Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above
- Abstract:
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The instability of DNA triplexes particularly at neutral pH and above severely limits their applications. Here, we demonstrate that the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs) resolves this problem. The stabilising effects are additive; multiple TO units produce nanomolar duplex binding and triplex stability can surpass that of the underlying duplex. In one example, a TFO containing three TO units increased the t...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 828.2KB)
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- Publisher copy:
- 10.1039/c8sc02418a
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Authors
Funding
+ European Union Horizon 2020 research and innovation programme
More from this funder
Grant:
Marie Sklodowska Curie agreement 642023 (H2020-MSCA-ITN-2014-
642023
Bibliographic Details
- Publisher:
- Royal Society of Chemistry Publisher's website
- Journal:
- Chemical Science Journal website
- Volume:
- 9
- Issue:
- 39
- Article number:
- 7681
- Publication date:
- 2018-08-01
- Acceptance date:
- 2018-07-30
- DOI:
- EISSN:
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2041-6539
- ISSN:
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2041-6520
- Source identifiers:
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935218
Item Description
- Pubs id:
-
pubs:935218
- UUID:
-
uuid:d79cbd11-6811-4778-9e7c-ff2743d78d39
- Local pid:
- pubs:935218
- Deposit date:
- 2018-11-01
Terms of use
- Copyright holder:
- Royal Society of Chemistry
- Copyright date:
- 2018
- Notes:
- © The Royal Society of Chemistry 2018. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
- Licence:
- CC Attribution (CC BY)
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