Genetic characterization of Pseudomonas fluorescens SBW25 rsp gene expression in the phytosphere and in vitro.

Journal article

The plant-colonizing Pseudomonas fluorescens strain SBW25 harbors a gene cluster (rsp) whose products show similarity to type III protein secretion systems found in plant and animal pathogens. Here we report a detailed analysis of the expression and regulation of the P. fluorescens rsp pathway, both in the phytosphere and in vitro. A combination of chromosomally integrated transcriptional reporter fusions, overexpressed regulatory genes, and specific mutants reveal that promoters controlling ...

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Authors: Jackson, R; Preston, G; Rainey, P

• Publication status: Published
• Journal: Journal of bacteriology
• Volume: 187
• Issue: 24
• Pages: 8477-8488
• Publication date: 2005-12-01
• DOI: 10.1128/jb.187.24.8477-8488.2005
• EISSN: 1098-5530
• ISSN: 0021-9193
• Language: English
• Keywords: Gene Expression; Pseudomonas fluorescens; Promoter Regions, Genetic; Base Sequence; Plant Leaves; Molecular Sequence Data; Arabidopsis; DNA-Binding Proteins; Biological Transport; Transcription, Genetic; Mutagenesis, Insertional; Artificial Gene Fusion; Green Fluorescent Proteins; Beta vulgaris; Culture Media; Colony Count, Microbial; Gene Expression Regulation, Bacterial; Plant Roots; Mutation; Gene Dosage; Bacterial Proteins; Genes, Regulator; Plants