Journal article
Characterisation of utrophin modulator SMT C1100 as a non-competitive inhibitor of firefly luciferase
- Abstract:
-
Firefly luciferase (FLuc) is a powerful tool for molecular and cellular biology, and popular in high-throughput screening and drug discovery. However, FLuc assays have been plagued with positive and negative artefacts due to stabilisation and inhibition by small molecules from a range of chemical classes. Here we disclose Phase II clinical compound SMT C1100 for the treatment of Duchenne muscular dystrophy as an FLuc inhibitor (KD of 0.40 ± 0.15 µM). Enzyme kinetic studies using SMT C1100 and...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Bioorganic Chemistry Journal website
- Volume:
- 94
- Article number:
- 103395
- Publication date:
- 2019-10-28
- Acceptance date:
- 2019-10-22
- DOI:
- EISSN:
-
1090-2120
- ISSN:
-
0045-2068
- Pmid:
-
31733898
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:1074776
- UUID:
-
uuid:d75fc108-26dc-4ec9-bf90-c8560e1be698
- Local pid:
- pubs:1074776
- Source identifiers:
-
1074776
- Deposit date:
- 2020-01-18
Terms of use
- Copyright holder:
- Wilkinson et al
- Copyright date:
- 2019
- Notes:
- © 2019 The Authors. Published by Elsevier Inc. This is an open access article published under CC BY 4.0.
- Licence:
- CC Attribution (CC BY)
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