CD4+ regulatory T cells generated in vitro with IFN-{gamma} and allogeneic APC inhibit transplant arteriosclerosis.

Journal article

We have developed a method to generate alloreactive regulatory T cells in vitro in the presence of interferon (IFN)-gamma and donor antigen presenting cells (APCs). We hypothesized that these IFN-gamma-conditioned T cells (Tcon) would reduce transplantation-associated arteriosclerosis. Tcon were generated from mouse (CBA.Ca, H-2(k)) CD4(+) T cells cultured in the presence of IFN-gamma for 14 days. These cultures were pulsed with bone marrow-derived B6 (H-2(b)) APC. 1 x 10(5) CD25(-)CD4(+) eff...

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Authors: Warnecke, G; Feng, G; Goto, R; Nadig, SN; Francis, R

• Publication status: Published
• Journal: American journal of pathology
• Volume: 177
• Issue: 1
• Pages: 464-472
• Publication date: 2010-07-01
• DOI: 10.2353/ajpath.2010.090292
• EISSN: 1525-2191
• ISSN: 0002-9440
• Language: English
• Keywords: Antigen-Presenting Cells; Mice, Inbred CBA; Transplantation Conditioning; Adoptive Transfer; T-Lymphocytes, Regulatory; CD4-Positive T-Lymphocytes; Transplantation, Homologous; Mice, Inbred C57BL; Mice; Animals; Aorta, Abdominal; Interferon-gamma; Arteriosclerosis