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Journal article

Identification of the (183)RWTNNFREY(191) region as a critical segment of matrix metalloproteinase 1 for the expression of collagenolytic activity.

Abstract:

Matrix metalloproteinase 1 (MMP-1) cleaves types I, II, and III collagen triple helices into (3/4) and (1/4) fragments. To understand the structural elements responsible for this activity, various lengths of MMP-1 segments have been introduced into MMP-3 (stromelysin 1) starting from the C-terminal end. MMP-3/MMP-1 chimeras and variants were overexpressed in Escherichia coli, folded from inclusion bodies, and isolated as zymogens. After activation, recombinant chimeras were tested for their a...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m004039200

Authors


Shimokawa, K More by this author
Dinakarpandian, D More by this author
Fields, GB More by this author
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Journal:
The Journal of biological chemistry
Volume:
275
Issue:
38
Pages:
29610-29617
Publication date:
2000-09-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:d6be4571-37fb-43de-a5ad-aa7860d7c647
Source identifiers:
227161
Local pid:
pubs:227161

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