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Rapid isolation of arabidopsis chloroplasts and their use for in vitro protein import assays

Abstract:

In vitro chloroplast protein import assays have been performed since the late 1970s, initially with plant species (e.g., pea and spinach) that readily provide an abundant source of starting material and also, subsequently, a good yield of chloroplasts for import assays. However, the sequencing of the Arabidopsis genome paved the way for an additional model system that is more amenable to genetic analysis, as a complement to the more biochemically orientated models such as pea and spinach. A p...

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Authors


Aronsson, H More by this author
Jarvis, RP More by this author
Journal:
Methods in Molecular Biology
Volume:
774
Pages:
281-305
Publication date:
2011
DOI:
ISSN:
1064-3745
URN:
uuid:d682b135-4a5a-4781-8951-c6692a34f834
Source identifiers:
411884
Local pid:
pubs:411884

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