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Optogenetic control of iPS cell‐derived neurons in 2D and 3D culture systems using channelrhodopsin‐2 expression driven by the synapsin‐1 and calcium‐calmodulin kinase II promoters

Abstract:

Development of an optogenetically controllable human neural network model in three‐dimensional (3D) cultures can provide an investigative system that is more physiologically relevant and better able to mimic aspects of human brain function. Light‐sensitive neurons were generated by transducing channelrhodopsin‐2 (ChR2) into human induced pluripotent stem cell (hiPSC) derived neural progenitor cells (Axol) using lentiviruses and cell‐type specific promoters. A mixed population of human iPSC‐de...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1002/term.2786

Authors


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ORCID:
0000-0002-2503-2338
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
ORCID:
0000-0002-0701-5040
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ORCID:
0000-0002-9055-1775
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science
Oxford college:
Linacre College
ORCID:
0000-0001-7613-6041
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ORCID:
0000-0001-9066-0181
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Publisher:
Wiley Publisher's website
Journal:
Journal of Tissue Engineering and Regenerative Redicine Journal website
Volume:
13
Issue:
3
Pages:
369-384
Publication date:
2019-01-30
Acceptance date:
2018-11-30
DOI:
EISSN:
1932-7005
ISSN:
1932-6254
Pubs id:
pubs:955104
URN:
uri:d66ade4d-3363-4d99-89f5-1c1185da2b8d
UUID:
uuid:d66ade4d-3363-4d99-89f5-1c1185da2b8d
Local pid:
pubs:955104

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