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Structure of the ultra-high-affinity colicin E2 DNase-Im2 complex

Abstract:

How proteins achieve high-affinity binding to a specific protein partner while simultaneously excluding all others is a major biological problem that has important implications for protein design. We report the crystal structure of the ultra-high-affinity protein-protein complex between the endonuclease domain of colicin E2 and its cognate immunity (Im) protein, Im2 (K d∼ 10 -15 M), which, by comparison to previous structural and biophysical data, provides unprecedented insight into how high ...

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Publisher copy:
10.1016/j.jmb.2012.01.019

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Journal:
Journal of Molecular Biology
Volume:
417
Issue:
1-2
Pages:
79-94
Publication date:
2012-03-16
DOI:
EISSN:
1089-8638
ISSN:
0022-2836
URN:
uuid:d62aa90d-34bc-4474-9a8c-d2837956a703
Source identifiers:
320755
Local pid:
pubs:320755

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