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Effective knockdown of Drosophila long noncoding RNAs by CRISPR interference

Abstract:

Long noncoding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient to address the role of lncRNA transcription from the transcript which has impeded analysis of their function. Using the minimal CRISPR interference (CRISPRi) system, we show that co-expression ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkw063

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More from this funder
Name:
European Research Council
Grant:
DARGENs, Project number 249869
Publisher:
Oxford University Press
Journal:
Nucleic Acids Research More from this journal
Volume:
44
Issue:
9
Article number:
e84
Publication date:
2016-01-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Pubs id:
pubs:597726
UUID:
uuid:d6245ac3-85bf-4b90-ba96-0500f2485ccc
Local pid:
pubs:597726
Source identifiers:
597726
Deposit date:
2016-01-27

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