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Journal article

Engineering cytochrome P450cam into an alkane hydroxylase

Abstract:

The haem monooxygenase cytochrome P450cam from Pseudomonas putida has been engineered into an alkane hydroxylase. Active site amino acid residues were substituted with residues that have bulkier and more hydrophobic side-chains. The residues F87, Y96, V247 and V396, which are further away from the haem, were targeted first for substitution in order to constrain the small alkanes n-butane and propane to bind closer to the haem. We found that just two mutations could increase the alkane oxidati...

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Publication status:
Published

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Publisher copy:
10.1039/b300869j

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Journal:
DALTON TRANSACTIONS
Issue:
11
Pages:
2133-2140
Publication date:
2003-01-01
DOI:
EISSN:
1477-9234
ISSN:
1477-9226
Source identifiers:
38654
Language:
English
Pubs id:
pubs:38654
UUID:
uuid:d5bcdf25-ece9-402f-a4e5-76ffa3a2da76
Local pid:
pubs:38654
Deposit date:
2012-12-19

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