Abolishment of the Tyr-15 inhibitory phosphorylation site on cdc2 reduces the radiation-induced G(2) delay, revealing a potential checkpoint in early mitosis.

Journal article

cdc2 is inactivated before mitosis by phosphorylation at its inhibitory sites, Thr-14 and Tyr-15. Irradiation prevents HeLa cells from completing the G(2)-M transition, and they arrest in G(2). Whereas phosphorylation at both of these sites occurs during the G(2) arrest, the individual role of each site in the G(2) delay has not previously been investigated. We have shown that the radiation-induced G(2) delay is preserved in wild-type or cdc2-AY-transfected cells (which retain Tyr-15); this d...

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Authors: Fletcher, L; Cheng, Y; Muschel, R

• Publication status: Published
• Journal: Cancer research
• Volume: 62
• Issue: 1
• Pages: 241-250
• Publication date: 2002-01-01
• EISSN: 1538-7445
• ISSN: 0008-5472
• Language: English
• Keywords: Nuclear Proteins; G2 Phase; Chromatin; Structure-Activity Relationship; Mutagenesis; Lamin Type B; Cyclin B; Transfection; Humans; Phosphorylation; Tyrosine; Hela Cells; Mitosis; CDC2 Protein Kinase; Cyclin B1; Binding Sites; Lamins