Evidence for a dual mechanism for IL-10 suppression of TNF-alpha production that does not involve inhibition of p38 mitogen-activated protein kinase or NF-kappa B in primary human macrophages.

Journal article

IL-10 is a potent anti-inflammatory cytokine and inhibitor of TNF-alpha production. The molecular pathways by which IL-10 inhibits TNF-alpha production are obscure, with diverse mechanisms having been published. In this study, a new approach has been taken for the study of human cells. Adenovirus was used to deliver TNF-alpha promoter-based luciferase reporter genes to primary human monocytic cells. The reporter genes were highly responsive to macrophage activation and appeared to mirror the ...

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Authors: Denys, A; Udalova, I; Smith, C; Williams, L; Ciesielski, C

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 168
• Issue: 10
• Pages: 4837-4845
• Publication date: 2002-05-01
• DOI: 10.4049/jimmunol.168.10.4837
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Immunosuppressive Agents; RNA, Messenger; Zymosan; Adenoviridae; Lipopolysaccharides; Cells, Cultured; Macrophages; Promoter Regions, Genetic; Tumor Necrosis Factor-alpha; 3' Untranslated Regions; Interleukin-10; 5' Untranslated Regions; Humans; Gene Expression Regulation; Enzyme Inhibitors; p38 Mitogen-Activated Protein Kinases; Enzyme Activation; RNA Stability; Genes, Reporter; Mitogen-Activated Protein Kinases; NF-kappa B