Evidence for the assembly of a bacterial tripartite multidrug pump with a stoichiometry of 3:6:3.

Journal article

The multiple transferable resistance (mTR) pump from Neisseria gonorrhoeae MtrCDE multidrug pump is assembled from the inner and outer membrane proteins MtrD and MtrE and the periplasmic membrane fusion protein MtrC. Previously we established that while there is a weak interaction of MtrD and MtrE, MtrC binds with relatively high affinity to both MtrD and MtrE. MtrD conferred antibiotic resistance only when it was expressed with MtrE and MtrC, suggesting that these proteins form a functional ...

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Authors: Janganan, T; Bavro, V; Zhang, L; Matak-Vinkovic, D; Barrera, N

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 286
• Issue: 30
• Pages: 26900-26912
• Publication date: 2011-07-01
• DOI: 10.1074/jbc.m111.246595
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Protein Structure, Quaternary; Membrane Transport Proteins; Drug Resistance, Multiple, Bacterial; Mutation, Missense; Multiprotein Complexes; Lipoproteins; Amino Acid Substitution; Protein Binding; Membrane Proteins; Bacterial Proteins; Bacterial Outer Membrane Proteins; Neisseria gonorrhoeae