- Abstract:
-
We have developed and validated a semi-automated fluorescent method of genotyping human leucocyte antigen (HLA)-DRB1 alleles, HLA-DRB1*01-16, by multiplex primer extension reactions. This method is based on the extension of a primer that anneals immediately adjacent to the single-nucleotide polymorphism with fluorescent dideoxynucleotide triphosphates (minisequencing), followed by analysis on an ABI Prism 3700 capillary electrophoresis instrument. The validity of the method was confirmed by g...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1111/j.1399-0039.2004.00241.x
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- Journal:
- Tissue antigens
- Volume:
- 64
- Issue:
- 1
- Pages:
- 88-95
- Publication date:
- 2004-07-05
- DOI:
- EISSN:
-
1399-0039
- ISSN:
-
0001-2815
- URN:
-
uuid:d552c1fa-f6a0-472a-87a9-2b6813dffc42
- Source identifiers:
-
108780
- Local pid:
- pubs:108780
- Copyright date:
- 2004
Journal article
A novel human leucocyte antigen-DRB1 genotyping method based on multiplex primer extension reactions.
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