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A novel human leucocyte antigen-DRB1 genotyping method based on multiplex primer extension reactions.

Abstract:

We have developed and validated a semi-automated fluorescent method of genotyping human leucocyte antigen (HLA)-DRB1 alleles, HLA-DRB1*01-16, by multiplex primer extension reactions. This method is based on the extension of a primer that anneals immediately adjacent to the single-nucleotide polymorphism with fluorescent dideoxynucleotide triphosphates (minisequencing), followed by analysis on an ABI Prism 3700 capillary electrophoresis instrument. The validity of the method was confirmed by g...

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Publication status:
Published

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Journal:
Tissue antigens
Volume:
64
Issue:
1
Pages:
88-95
Publication date:
2004-07-05
DOI:
EISSN:
1399-0039
ISSN:
0001-2815
URN:
uuid:d552c1fa-f6a0-472a-87a9-2b6813dffc42
Source identifiers:
108780
Local pid:
pubs:108780

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