Journal article icon

Journal article

p19-mediated enrichment and detection of siRNAs.

Abstract:

p19 is an RNA binding protein originally isolated from the Carnation Italian ring-spot virus (CIRV). It has been shown that p19 is a plant RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity. A bifunctional p19 fusion protein, with an N-terminal maltose binding protein (MBP) and a C-terminal chitin binding domain (CBD) allows protein purification and binding of p19 to chitin magnetic beads via the chitin binding domain. The fusion p19 protein recognizes and bi...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1007/978-1-4939-0931-5_9

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.) More from this journal
Volume:
1173
Pages:
99-111
Publication date:
2014-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:469535
UUID:
uuid:d54a8ffe-f548-42c6-b070-8c299212c1f6
Local pid:
pubs:469535
Source identifiers:
469535
Deposit date:
2014-06-30

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP