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Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage)

Abstract:

The total thiols in a medium containing authentic biological sample (articular cartilage) has been determined electrochemically. The detection strategy utilises the electrochemical adaptation of the Ellman's reaction process via the electrochemically initiated reaction of thiols with diethyl-p-phenylenediamine. The strategy has been examined over a biologically significant pH range and the behaviour observed rationalised. The detection of thiols within the biological sample is shown to procee...

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Publication status:
Published

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Authors


Nekrassova, O More by this author
Lawrence, NS More by this author
Urban, JPG More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Journal:
INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL and ANALYTICAL CHEMISTRY
Volume:
42
Issue:
4
Pages:
739-743
Publication date:
2003-04-05
ISSN:
0376-4710
URN:
uuid:d3adc38c-c1ac-4d32-a454-f3361f9e1fa7
Source identifiers:
38568
Local pid:
pubs:38568
Language:
English

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