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Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage)

Abstract:

The total thiols in a medium containing authentic biological sample (articular cartilage) has been determined electrochemically. The detection strategy utilises the electrochemical adaptation of the Ellman's reaction process via the electrochemically initiated reaction of thiols with diethyl-p-phenylenediamine. The strategy has been examined over a biologically significant pH range and the behaviour observed rationalised. The detection of thiols within the biological sample is shown to procee...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL and ANALYTICAL CHEMISTRY More from this journal
Volume:
42
Issue:
4
Pages:
739-743
Publication date:
2003-04-01
ISSN:
0376-4710
Language:
English
UUID:
uuid:d3adc38c-c1ac-4d32-a454-f3361f9e1fa7
Local pid:
pubs:38568
Source identifiers:
38568
Deposit date:
2012-12-19

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