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The role of carbonic anhydrase 9 in regulating extracellular and intracellular ph in three-dimensional tumor cell growths.

Abstract:

We have studied the role of carbonic anhydrase 9 (CA9), a cancer-associated extracellular isoform of the enzyme carbonic anhydrase in multicellular spheroid growths (radius of approximately 300 microm) of human colon carcinoma HCT116 cells. Spheroids were transfected with CA9 (or empty vector) and imaged confocally (using fluorescent dyes) for both intracellular pH (pH(i)) and pH in the restricted extracellular spaces (pH(e)). With no CA9 expression, spheroids developed very low pH(i) (approx...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m109.006478

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics, BHF Centre of Research Excellence
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics, BHF Centre of Research Excellence
Role:
Author
Journal:
The Journal of biological chemistry
Volume:
284
Issue:
30
Pages:
20299-20310
Publication date:
2009-07-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:d2a26f4e-089c-4df3-a3eb-d644d3df0fbb
Source identifiers:
106417
Local pid:
pubs:106417

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