The role of carbonic anhydrase 9 in regulating extracellular and intracellular ph in three-dimensional tumor cell growths.

Journal article

We have studied the role of carbonic anhydrase 9 (CA9), a cancer-associated extracellular isoform of the enzyme carbonic anhydrase in multicellular spheroid growths (radius of approximately 300 microm) of human colon carcinoma HCT116 cells. Spheroids were transfected with CA9 (or empty vector) and imaged confocally (using fluorescent dyes) for both intracellular pH (pH(i)) and pH in the restricted extracellular spaces (pH(e)). With no CA9 expression, spheroids developed very low pH(i) (approx...

Expand abstract

Authors: Swietach, P; Patiar, S; Supuran, C; Harris, A; Vaughan-Jones, R

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 284
• Issue: 30
• Pages: 20299-20310
• Publication date: 2009-07-01
• DOI: 10.1074/jbc.m109.006478
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Gene Expression; Spheroids, Cellular; Hydrogen-Ion Concentration; Tumor Cells, Cultured; Humans; Acids; Carbonic Anhydrases; Colonic Neoplasms; Antigens, Neoplasm; Cell Line, Tumor; Carbon Dioxide; Carcinoma; Bicarbonates; Fluorescein