Inhibition of glucose-stimulated insulin release by alpha 2-adrenoceptor activation is parallelled by both a repolarization and a reduction in cytoplasmic free Ca2+ concentration.

Journal article

Effects of the alpha 2-adrenergic agonist clonidine on insulin release, membrane potential, and cytoplasmic free Ca2+ concentration ([Ca2+]i) were investigated using pancreatic beta-cells isolated from obese hyperglycemic mice. Addition of 2 microM clonidine promptly inhibited glucose-stimulated insulin release, an effect accompanied by a lowering in both membrane potential and [Ca2+]i. Within minutes, the effect on Ca2+ was partly reversed, [Ca2+]i attaining a new level, although still signi...

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Authors: Nilsson, T; Arkhammar, P; Rorsman, P; Berggren, P

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 263
• Issue: 4
• Pages: 1855-1860
• Publication date: 1988-02-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Membrane Potentials; Clonidine; Aminoquinolines; Calcium; Female; Yohimbine; Male; Adrenergic alpha-Agonists; Potassium; Ouabain; Insulin; Diazoxide; Cytoplasm; Glucose; Animals; Mice