Journal article icon

Journal article

Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing

Abstract:

Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian OROV lineage was identified from metagenomic sequencing data. We report the optimisation of an qRT-PCR assay for the Ecuadorian OROV lineage, which subsequently identified a further five cases in a ...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Files:
Publisher copy:
10.1371/journal.pntd.0007897

Authors


More by this author
Role:
Author
ORCID:
0000-0002-0279-3900
More from this funder
Name:
European Commission
Grant:
1341560
More from this funder
Name:
Medical Research Council
Grant:
MC_PC_16028
Publisher:
Public Library of Science
Journal:
PLoS Neglected Tropical Diseases More from this journal
Volume:
14
Issue:
1
Pages:
e0007897-
Publication date:
2020-01-01
Acceptance date:
2019-10-31
DOI:
EISSN:
1935-2735
Language:
English
Pubs id:
1084349
Local pid:
pubs:1084349
Deposit date:
2020-02-04

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP