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Nanoscopy with more than 100,000 'doughnuts'.

Abstract:
We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 μm × 100 μm-sized fields of view in <1 s using 116,000 such doughnuts.

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Publisher copy:
10.1038/nmeth.2556

Authors


Chmyrov, A More by this author
Chmyrov, A More by this author
Grotjohann, T More by this author
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Journal:
Nature methods
Volume:
10
Issue:
8
Pages:
737-740
Publication date:
2013-08-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:d0e592de-4df1-450e-8c91-086a7edb0923
Source identifiers:
411231
Local pid:
pubs:411231

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