Nanomapping of CD1d-glycolipid complexes on THP1 cells by using simultaneous topography and recognition imaging.

Journal article

CD1d molecule, a monomorphic major histocompatibility complex class I-like molecule, presents different types of glycolipids to invariant natural killer T (iNKT) cells that play an important role in immunity to infection and tumors, as well as in regulating autoimmunity. Here, we present simultaneous topography and recognition imaging (TREC) analysis to detect density, distribution and localization of single CD1d molecules on THP1 cells that were loaded with different glycolipids. TREC was co...

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Authors: Duman, M; Chtcheglova, L; Zhu, R; Bozna, B; Polzella, P

• Publication status: Published
• Journal: Journal of molecular recognition : JMR
• Volume: 26
• Issue: 9
• Pages: 408-414
• Publication date: 2013-09-01
• DOI: 10.1002/jmr.2282
• EISSN: 1099-1352
• ISSN: 0952-3499
• Language: English
• Keywords: Binding Sites; Galactosylceramides; Glycolipids; Microscopy, Atomic Force; Cell Line, Tumor; Antigens, CD1d; Single-Cell Analysis; Cell Membrane; Humans