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Towards high-throughput flim for protein-protein interaction screening of live cells and tissue microarrays

Abstract:

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scri...

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Publication status:
Published

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Publisher copy:
10.1109/ISBI.2008.4541006

Authors


Host title:
2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4
Pages:
356-359
Publication date:
2008-01-01
DOI:
ISSN:
1945-7928
ISBN:
9781424420025
Keywords:
Pubs id:
pubs:73001
UUID:
uuid:d067185a-d95c-4470-9a3d-291b9a957e2d
Local pid:
pubs:73001
Source identifiers:
73001
Deposit date:
2012-12-19

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