Journal article icon

Journal article

DNA-templated protein arrays for single-molecule imaging.

Abstract:

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this tech...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1021/nl1037769

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Role:
Author
Expand authors...
Journal:
Nano letters
Volume:
11
Issue:
2
Pages:
657-660
Publication date:
2011-02-05
DOI:
EISSN:
1530-6992
ISSN:
1530-6984
URN:
uuid:d019b834-a8ad-45d5-a842-6d8fc0f04d0d
Source identifiers:
112503
Local pid:
pubs:112503

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP