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Mapping protein interactions of sodium channel NaV1.7 using epitope‐tagged gene‐targeted mice

Abstract:

The voltage-gated sodium channel NaV1.7 plays a critical role in pain pathways. We generated an epitope-tagged NaV1.7 mouse that showed normal pain behaviours to identify channel-interacting proteins. Analysis of NaV1.7 complexes affinity-purified under native conditions by mass spectrometry revealed 267 proteins associated with Nav1.7 in vivo The sodium channel β3 (Scn3b), rather than the β1 subunit, complexes with Nav1.7, and we demonstrate an interaction between collapsing-response mediato...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.15252/embj.201796692

Authors


Kanellopoulos, AH More by this author
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
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ORCID:
0000-0002-5751-178X
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Grant:
Collaborative Award 200183/Z/15/Z (to J.W., J.Z., J.C. and S.G.)
Publisher:
EMBO Press Publisher's website
Journal:
EMBO Journal Journal website
Volume:
37
Issue:
3
Pages:
427-445
Publication date:
2018-01-15
Acceptance date:
2017-12-05
DOI:
ISSN:
1460-2075
Pubs id:
pubs:820915
URN:
uri:d0199756-b750-4b71-9b11-017bad0a0dc5
UUID:
uuid:d0199756-b750-4b71-9b11-017bad0a0dc5
Local pid:
pubs:820915

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