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Journal article

Microhomologies are prevalent at Cas9-induced larger deletions

Abstract:

The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger deletions are prevalent at multiple distinct loci on different chromosomes, in cultured cells and mouse embryos alike. We observed a high frequency of microhomologies at larger deletion br...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkz459

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
RDM Clinical Laboratory Sciences
Oxford college:
Hertford College
Role:
Author
ORCID:
0000-0003-3668-1859
Publisher:
Oxford University Press
Journal:
Nucleic Acids Research More from this journal
Publication date:
2019-05-25
Acceptance date:
2019-05-14
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Pubs id:
pubs:1008924
UUID:
uuid:d001fd49-447a-409c-a861-674f8a47a7c3
Local pid:
pubs:1008924
Source identifiers:
1008924
Deposit date:
2019-06-07

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