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Choosing the right label for single-molecule tracking in live bacteria: side-by-side comparison of photoactivatable fluorescent protein and Halo tag dyes

Abstract:

Visualizing and quantifying molecular motion and interactions inside living cells provides crucial insight into the mechanisms underlying cell function. This has been achieved by super-resolution localization microscopy and single-molecule tracking in conjunction with photoactivatable fluorescent proteins (PA-FPs). An alternative labelling approach relies on genetically-encoded protein tags with cell-permeable fluorescent ligands which are brighter and less prone to photobleaching than fluore...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1088/1361-6463/aaf255

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Oxford college:
Linacre College
Role:
Author
ORCID:
0000-0003-2384-0857
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
ORCID:
0000-0002-3579-0888
More from this funder
Funding agency for:
Uphoff, S
More from this funder
Funding agency for:
Uphoff, S
Publisher:
IOP Publishing Publisher's website
Journal:
Journal of Physics D: Applied Physics Journal website
Volume:
52
Pages:
064002
Publication date:
2018-12-06
Acceptance date:
2018-11-20
DOI:
EISSN:
1361-6463
ISSN:
0022-3727
Pubs id:
pubs:951429
URN:
uri:cfefefdf-74a7-44c2-9075-57d96472f39a
UUID:
uuid:cfefefdf-74a7-44c2-9075-57d96472f39a
Local pid:
pubs:951429

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