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Journal article

Optimising ovine cerebrospinal fluid preparation for two-dimensional gel electrophoresis.

Abstract:

Biomarkers for neurodegenerative disorders are potentially present in cerebrospinal fluid (CSF) and can be detected using proteomic technologies. Since CSF is high in salt and low in protein, its study by proteomic methods requires appropriate sample preparation. In this study, we applied four different sample treatments to the same ovine CSF sample. Precipitation with acetone or using a 2-D Clean-Up Kit (GE Healthcare BioSciences, Little Chalfont, UK) preserved more proteins, and produced mo...

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Publication status:
Published

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Publisher copy:
10.1002/pmic.200500623

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Proteomics More from this journal
Volume:
6
Issue:
10
Pages:
3170-3175
Publication date:
2006-05-01
DOI:
EISSN:
1615-9861
ISSN:
1615-9853
Language:
English
Keywords:
Pubs id:
pubs:229627
UUID:
uuid:cf9252b8-20ea-4118-85d6-7618853c4f75
Local pid:
pubs:229627
Source identifiers:
229627
Deposit date:
2013-11-16

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