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Contribution of glutamate dehydrogenase to mitochondrial glutamate metabolism studied by (13)C and (31)P nuclear magnetic resonance.

Abstract:

The relative contribution of glutamate dehydrogenase (GDH) and the aminotransferase activity to mitochondrial glutamate metabolism was investigated in dilute suspensions of purified mitochondria from potato (Solanum tuberosum) tubers. Measurements of glutamate-dependent oxygen consumption by mitochondria in different metabolic states were complemented by novel in situ NMR assays of specific enzymes that metabolize glutamate. First, a new assay for aminotransferase activity, based on the excha...

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Publication status:
Published

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Publisher copy:
10.1093/jexbot/52.354.37

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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
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Journal:
Journal of experimental botany
Volume:
52
Issue:
354
Pages:
37-45
Publication date:
2001-01-01
DOI:
EISSN:
1460-2431
ISSN:
0022-0957
Source identifiers:
37703
Language:
English
Keywords:
Pubs id:
pubs:37703
UUID:
uuid:cf36e64e-3677-4e23-8783-d6146c6883b6
Local pid:
pubs:37703
Deposit date:
2012-12-19

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