Development of sensitive immunoassays for free and total human glandular kallikrein 2.

Journal article

BACKGROUND: Free and total human kallikrein 2 (hK2) might improve the discrimination between prostate cancer and benign prostatic hyperplasia. Concentrations of hK2 are 100-fold lower than concentrations of prostate-specific antigen (PSA); therefore, an hK2 assay must have a low detection limit and good specificity. METHODS: PSA- and hK2-specific monoclonal antibodies were used in solid-phase, two-site immunofluorometric assays to detect free and total hK2. The total hK2 assay used PSA-speci...

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Authors: Väisänen, V; Eriksson, S; Ivaska, K; Lilja, H; Nurmi, M

• Journal: Clinical chemistry
• Volume: 50
• Issue: 9
• Pages: 1607-1617
• Publication date: 2004-09-01
• DOI: 10.1373/clinchem.2004.035253
• EISSN: 1530-8561
• ISSN: 0009-9147
• Language: English
• Keywords: Antibody Specificity; Prostatic Hyperplasia; Humans; Antibodies, Monoclonal; Mice; Adult; Middle Aged; Aged; Animals; Mice, Inbred BALB C; Prostatic Neoplasms; Reproducibility of Results; Male; Tumor Markers, Biological; Aged, 80 and over; Diagnosis, Differential; Fluoroimmunoassay; Tissue Kallikreins; Epitope Mapping; Prostate-Specific Antigen