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Journal article

Preparation of high-quality next-generation sequencing libraries from picogram quantities of target DNA.

Abstract:

New sequencing technologies can address diverse biomedical questions but are limited by a minimum required DNA input of typically 1 μg. We describe how sequencing libraries can be reproducibly created from 20 pg of input DNA using a modified transpososome-mediated fragmentation technique. Resulting libraries incorporate in-line bar-coding, which facilitates sample multiplexes that can be sequenced using Illumina platforms with the manufacturer's sequencing primer. We demonstrate this techniqu...

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Publication status:
Published

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Publisher copy:
10.1101/gr.124016.111

Authors


Journal:
Genome research More from this journal
Volume:
22
Issue:
1
Pages:
125-133
Publication date:
2012-01-01
DOI:
EISSN:
1549-5469
ISSN:
1088-9051
Language:
English
Keywords:
Pubs id:
pubs:206006
UUID:
uuid:ce286f53-d669-4acf-ad6a-4c641a09b618
Local pid:
pubs:206006
Source identifiers:
206006
Deposit date:
2012-12-19

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