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Direct measurement of glutathione in epidermal cells of intact Arabidopsis roots by two-photon laser scanning microscopy.

Abstract:

Two-photon laser scanning microscopy (TPLSM) was used to directly measure glutathione (GSH) as its fluorescent glutathione S-bimane conjugate (GSB) in developing root hair cells (trichoblasts) and non-root hair cells (atrichoblasts) of intact Arabidopsis roots. In comparison to confocal microscopy, TPLSM showed more detail deep within the tissue with less signal attenuation. The total level of GSB labelling reached a plateau after 60 min in both trichoblasts and atrichoblasts, reflecting depl...

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Publication status:
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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
Journal:
Journal of microscopy More from this journal
Volume:
198
Issue:
Pt 3
Pages:
174-181
Publication date:
2000-06-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Language:
English
Keywords:
Pubs id:
pubs:31694
UUID:
uuid:ce0708ee-225e-4aa7-a2e1-0bd48ce52381
Local pid:
pubs:31694
Source identifiers:
31694
Deposit date:
2012-12-19

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