Synthetic oligonucleotide cocktails as probes for detection of human parvovirus B19.

Journal article

A cocktail of 10 oligonucleotides selected at intervals along the length of the genome of human parvovirus B19 was labelled enzymically with digoxigenin and chemically with either digoxigenin (DIG) or dinitrophenyl (DNP). Chemical labelling was easier and more practical for the production of large quantities of probe. Pools labelled with either digoxigenin or DNP could detect 10 fg of B19 DNA in a dot blot reaction using an alkaline phosphatase antibody conjugate and colorimetric detection. F...

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Authors: Cubie, H; Grzybowski, J; da Silva, C; Duncan, L; Brown, T

• Publication status: Published
• Journal: Journal of virological methods
• Volume: 53
• Issue: 1
• Pages: 91-102
• Publication date: 1995-05-01
• DOI: 10.1016/0166-0934(94)00179-k
• EISSN: 1879-0984
• ISSN: 0166-0934
• Language: English
• Keywords: Molecular Sequence Data; Dinitrobenzenes; Sensitivity and Specificity; Oligonucleotide Probes; DNA, Viral; Hydrops Fetalis; Base Sequence; Digoxigenin; Humans; Parvovirus B19, Human; In Situ Hybridization