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Synthetic oligonucleotide cocktails as probes for detection of human parvovirus B19.

Abstract:

A cocktail of 10 oligonucleotides selected at intervals along the length of the genome of human parvovirus B19 was labelled enzymically with digoxigenin and chemically with either digoxigenin (DIG) or dinitrophenyl (DNP). Chemical labelling was easier and more practical for the production of large quantities of probe. Pools labelled with either digoxigenin or DNP could detect 10 fg of B19 DNA in a dot blot reaction using an alkaline phosphatase antibody conjugate and colorimetric detection. F...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Journal of virological methods More from this journal
Volume:
53
Issue:
1
Pages:
91-102
Publication date:
1995-05-01
DOI:
EISSN:
1879-0984
ISSN:
0166-0934
Language:
English
Keywords:
Pubs id:
pubs:400081
UUID:
uuid:cdcee1a8-5f20-4962-9e32-b7bb2dc122d3
Local pid:
pubs:400081
Source identifiers:
400081
Deposit date:
2013-11-16

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