Journal article icon

Journal article

Ligand supplementation as a method to increase soluble heterologous protein production.

Abstract:

Ligand interactions are central to enzyme or receptor function, constituting a cornerstone in biochemistry and pharmacology. Here we discuss a ligand application that can be exploited to significantly increase the proportion of recombinant protein expressed in soluble form, by including ligands during the culture. Provided that a sufficiently soluble, cell-permeable and avid ligand is available, one can use it to stabilize nascently synthesized proteins, and in this manner promote solubility ...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1586/14789450.5.1.137

Authors


Oppermann, U More by this author
Journal:
Expert review of proteomics
Volume:
5
Issue:
1
Pages:
137-143
Publication date:
2008-02-05
DOI:
EISSN:
1744-8387
ISSN:
1478-9450
URN:
uuid:cd7b6d4f-53ff-497f-b59e-5633b5dba8bd
Source identifiers:
108446
Local pid:
pubs:108446

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP