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Selection and Characterization of a Nanobody Biosensor of GTP-Bound RHO Activities

Abstract:

RHO (Ras HOmologous) GTPases are molecular switches that activate, in their state bound to Guanosine triphosphate (GTP), key signaling pathways, which involve actin cytoskeleton dynamics. Previously, we selected the nanobody RH12, from a synthetic phage display library, which binds the GTP-bound active conformation of RHOA (Ras Homologous family member A). However, when expressed as an intracellular antibody, its blocking effect on RHO signaling led to a loss of actin fibers, which in...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3390/antib8010008

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Publisher:
MDPI Publisher's website
Journal:
Antibodies Journal website
Volume:
8
Issue:
1
Article number:
8
Publication date:
2019-01-09
Acceptance date:
2018-12-20
DOI:
EISSN:
2073-4468
Source identifiers:
981412
Language:
English
Keywords:
Pubs id:
pubs:981412
UUID:
uuid:cd610386-8363-4ccc-892f-04dd3d97070d
Local pid:
pubs:981412
Deposit date:
2019-03-12

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