Journal article
Selection and Characterization of a Nanobody Biosensor of GTP-Bound RHO Activities
- Abstract:
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RHO (Ras HOmologous) GTPases are molecular switches that activate, in their state bound to Guanosine triphosphate (GTP), key signaling pathways, which involve actin cytoskeleton dynamics. Previously, we selected the nanobody RH12, from a synthetic phage display library, which binds the GTP-bound active conformation of RHOA (Ras Homologous family member A). However, when expressed as an intracellular antibody, its blocking effect on RHO signaling led to a loss of actin fibers, which in...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 1.5MB)
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- Publisher copy:
- 10.3390/antib8010008
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Authors
Funding
Bibliographic Details
- Publisher:
- MDPI Publisher's website
- Journal:
- Antibodies Journal website
- Volume:
- 8
- Issue:
- 1
- Article number:
- 8
- Publication date:
- 2019-01-09
- Acceptance date:
- 2018-12-20
- DOI:
- EISSN:
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2073-4468
- Source identifiers:
-
981412
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:981412
- UUID:
-
uuid:cd610386-8363-4ccc-892f-04dd3d97070d
- Local pid:
- pubs:981412
- Deposit date:
- 2019-03-12
Terms of use
- Copyright holder:
- Keller, L et al
- Copyright date:
- 2019
- Notes:
- © 2019 Keller, L et al. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
- Licence:
- CC Attribution (CC BY)
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