Axon routing at the optic chiasm after enzymatic removal of chondroitin sulfate in mouse embryos.

Journal article

The effects of removing chondroitin sulfate from chondroitin sulfate proteoglycan molecules on guidance of retinal ganglion cell axons at the optic chiasm were investigated in a brain slice preparation of mouse embryos of embryonic day 13 to 15. Slices were grown for 5 hours and growth of dye-labeled axons was traced through the chiasm. After continuous enzymatic digestion of the chondroitin sulfate proteoglycans with chondroitinase ABC, which removes the glycosaminoglycan chains, navigation ...

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Authors: Chung, K; Taylor, J; Shum, D; Chan, S

• Journal: Development (Cambridge, England)
• Volume: 127
• Issue: 12
• Pages: 2673-2683
• Publication date: 2000-06-01
• EISSN: 1477-9129
• ISSN: 0950-1991
• Language: English
• Keywords: Animals; Embryonic and Fetal Development; Retinal Ganglion Cells; Mice; Axons; Diencephalon; Chondroitin Sulfates; Chondroitin Sulfate Proteoglycans; Gestational Age; Optic Chiasm; Mice, Inbred C57BL; Chondroitin ABC Lyase