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A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization.

Abstract:

Crystallization trials at the Division of Structural Biology in Oxford are now almost exclusively carried out using a high-throughput workflow implemented in the Oxford Protein Production Facility. Initial crystallization screening is based on nanolitre-scale sitting-drop vapour-diffusion experiments (typically 100 nl of protein plus 100 nl of reservoir solution per droplet) which use standard crystallization screening kits and 96-well crystallization plates. For 294 K crystallization trials ...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444905007808

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Acta crystallographica. Section D, Biological crystallography More from this journal
Volume:
61
Issue:
Pt 6
Pages:
651-657
Publication date:
2005-06-01
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Language:
English
Keywords:
Pubs id:
pubs:1631
UUID:
uuid:cc9a4769-bb6f-4c2d-a2f0-ef84bf269d04
Local pid:
pubs:1631
Source identifiers:
1631
Deposit date:
2012-12-19

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