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Isolation and preparation of chloroplasts from Arabidopsis thaliana plants.

Abstract:

A major area of research in the postgenomic era has been the proteomic analysis of various subcellular and suborganellar compartments. The success of these studies is to a large extent dependent upon efficient protocols for the preparation of highly pure organelles or suborganellar components. Here we describe a simple, rapid, and low-cost method for isolating a high yield of Arabidopsis chloroplasts. The method can readily be applied to wild-type plants and different mutants, and at differen...

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
425
Pages:
171-186
Publication date:
2008-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:cc9582eb-e9aa-4e8e-8893-b30cbffb7a07
Source identifiers:
411882
Local pid:
pubs:411882

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