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An optical method for the determination of platelet count in platelet samples contaminated with red blood cells.

Abstract:

We present a simple photometric method to determine the total concentration of platelets present in a sample independently of red blood cell concentration. Standard optical density curves for platelet samples ranging in concentration from 0 to 1.5 x 10(9) cells/ml and contaminated with red blood cells ranging in concentration from 0 to 0.03 x 10(9) cells/ml are determined. A study of the absorbance spectra of red blood cells and platelets suggests that by calculating the absorbance difference...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science, Biomedical Research Centre
Journal:
Journal of biochemical and biophysical methods
Volume:
24
Issue:
3-4
Pages:
215-223
Publication date:
1992-06-05
DOI:
EISSN:
0165-022X
ISSN:
0165-022X
URN:
uuid:cc6f763f-13f6-44d7-98d4-7994ba6ee4f1
Source identifiers:
61690
Local pid:
pubs:61690
Language:
English
Keywords:

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