Journal article icon

Journal article

Strong cation exchange-based fractionation of Lys-N-generated peptides facilitates the targeted analysis of post-translational modifications.

Abstract:

In proteomics multi-dimensional fractionation techniques are widely used to reduce the complexity of peptide mixtures subjected to mass spectrometric analysis. Here, we describe the sequential use of strong cation exchange and reversed phase liquid chromatography in the separation of peptides generated by a relatively little explored metallo-endopeptidase with Lys-N cleavage specificity. When such proteolytic peptides are subjected to low-pH strong cation exchange we obtain fractionation prof...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1074/mcp.m800285-mcp200

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Molecular and cellular proteomics : MCP More from this journal
Volume:
8
Issue:
1
Pages:
190-200
Publication date:
2009-01-01
DOI:
EISSN:
1535-9484
ISSN:
1535-9476
Language:
English
Keywords:
Pubs id:
pubs:384601
UUID:
uuid:cc051692-defb-4245-942f-1658483d273f
Local pid:
pubs:384601
Source identifiers:
384601
Deposit date:
2013-11-16

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP