A membrane-distal segment of the integrin alpha IIb cytoplasmic domain regulates integrin activation.

Journal article

Previous evidence suggests that interactions between integrin cytoplasmic domains regulate integrin activation. We have constructed and validated recombinant structural mimics of the heterodimeric alpha(IIb)beta(3) cytoplasmic domain. The mimics elicited polyclonal antibodies that recognize a combinatorial epitope(s) formed in mixtures of the alpha(IIb) and beta(3) cytoplasmic domains but not present in either isolated tail. This epitope(s) is present within intact alpha(IIb)beta(3), indicati...

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Authors: Ginsberg, M; Yaspan, B; Forsyth, J; Ulmer, T; Campbell, I

• Journal: Journal of biological chemistry
• Volume: 276
• Issue: 25
• Pages: 22514-22521
• Publication date: 2001-06-01
• DOI: 10.1074/jbc.m101915200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Cell Membrane; Molecular Sequence Data; Enzyme-Linked Immunosorbent Assay; Recombinant Proteins; Cytoplasm; Integrins; Amino Acid Sequence